Variation of photostability of DNA-sensitive styrylcyanine dyes caused by N-alkyl functionalization

• DOI: 10.7124/bc.000a28
• Publication Date: 2020-07-16T15:33:00Z
• Authors: Y. V. Snihirova; M. Yu. Losytskyy; D. V. Kryvorotenko; M. V. Kuperman; O. V. Moshynets; S. M. Yarmoluk; A. Mokhir; V. B. Kovalska
• Keywords: Surface Modification

Aim. Synthesis and characterization of benzothiazole-based styrylcyanines functionalized by different N-alkyl tail groups as potential photostable probes for nucleic acids (NA) detection.Methods.Chemical synthesis, absorption fluorescence spectroscopy, irradiation with a visible light source, confocal microscopy.Results.Styrylcyanines are weakly fluorescent when free, but in the presence NA they show emission intensity increase up to 83 times; most pronounced was observed dyes Sbt1 Sbt3 bearing positively charged group.Photostability non-bound is low while binding DNA results strong their photostability.Dyes uncharged negatively more slightly affects photostability.This also due DNA.Sbt1 brightly stains cytoplasm nuclear components (probably nucleoli) mesenchymal stem cells; therefore this dye may be used visualize cytoplasmic RNA clusters.Conclusions.Variation chemical nature group allows design photostability.Functionalized styrylcyanine detection solution visualization RNA-containing microscopy.