Circular RNA CircEZH2 Suppresses Transmissible Gastroenteritis Coronavirus-induced Opening of Mitochondrial Permeability Transition Pore via Targeting MiR-22 in IPEC-J2

• DOI: 10.7150/ijbs.36532
• Publication Date: 2019-08-13T06:41:23Z
• Authors: Xiaomin Zhao; Xuelian Ma; Jianxiong Guo; Mi Mi; Kaili Wang; Chuyi Zhang; Xiaoyi Tang; Lingling Chang; Yong Huang; Dewen Tong
• Keywords: MPTP; Coronavirus

Transmissible gastroenteritis (TGE) is a contagious and infectious disease that characterized by severe vomiting diarrhea of swine , especially piglet, caused transmissible coronavirus (TGEV) .TGEV infection provokes mitochondrial damage porcine intestinal epthelial cell (IPEC), which responsible for inflammation death.In our previous study, we have demonstrated circular RNA circEZH2 was down-regulated during TGEV promoted the activation nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) via targeting miR-22 in epithelial line (IPEC-J2).Activation NF-κB an important damage.Mitochondrial permeability transition pore (mPTP) opening key reason damage.So, speculate may regulate TGEV-induced mPTP NF-kB pathway.In present found IPEC-J2 occured suppressed attaching miR-22.Hexokinase 2 (HK2) interleukin 6 (IL-6) were identified as targets miR-22.Silencing HK2 enhanced opening, while no effect on pathway.Silencing IL-6 inhibited pathway.Inhibitor increased opening.The data revealed regulated two pathways: circEZH2/miR-22/HK2 axis circEZH2/miR-22/IL-6/NF-κB axis.