B19, a Novel Monocarbonyl Analogue of Curcumin, Induces Human Ovarian Cancer Cell Apoptosis via Activation of Endoplasmic Reticulum Stress and the Autophagy Signaling Pathway

• DOI: 10.7150/ijbs.5711
• Publication Date: 2013-09-04T20:04:57Z
• Authors: Wanglei Qu; Jian Xiao; Hongyu Zhang; Qiong Chen; Zhouguang Wang; Hongxue Shi; Liang Gong; Jianqiang Chen; Yanlong Liu; Risheng Cao; Jieqiang Lv
• Keywords: Viability assay

Background:The unfolded protein response, autophagy and endoplasmic reticulum (ER) stress-induced apoptosis regulate tumor cell fate have become novel signaling targets for the development of cancer therapeutic drugs.Curcumin has been used to treat several different cancers, including ovarian cancer, in clinical trials research; however, role ER stress effects curcumin new analogues remains unclear.Methods: Cell viability was determined using MTT assay.Apoptosis detected flow cytometry with PI/Annexin V-FITC staining.The expression levels stress-and autophagy-related proteins were analyzed by western blotting.The activation immunofluorescence staining.Results: We demonstrated that B19 induced HO8910 a dose-responsive manner.We also this effect associated corresponding increases series key components UPR stress-mediated pathways, followed caspase 3 cleavage activation.We observed treatment cells.The inhibition 3-methyladenine (3-MA) increased intracellular misfolded proteins, which enhanced apoptosis.Conclusions: Our data indicate may play is analogue an epithelial line can increase analogue-induced inducing severe stress.