In vivo Antitumor Effect of an HPV-specific Promoter driving IL-12 Expression in an HPV 16-positive Murine Model of Cervical Cancer

• DOI: 10.7150/jca.15536
• Publication Date: 2016-11-09T20:12:04Z
• Authors: Victor Hugo Bermú...; Geny Fierros-Zarate; Celina García-Mel...; Juan Manuel Alcoc...; Ausencio Morales-...; Oscar Peralta-Zar...; Kirvis Torres-Poveda; Ana Isabel Burgue...; Eva Hernández-Már...; Vicente Madrid-Ma...
• Keywords: Bovine papillomavirus

Human papillomavirus (HPV) is a DNA virus that infects epithelial cells and has been implicated in the development of cervical cancer.Few therapeutic strategies have designed for treatment intraepithelial neoplasia, precursor cancer.In these early stages, HPV E2 protein most important viral factor involved gene expression plays crucial roles during vegetative cycle cells.Papillomavirus binds specifically to palindromic ACCN 6 GGT sequences, referred as binding sites (E2BS), which are concentrated within long control region, responsible regulation protein's expression.Here, we consider E2BS candidate sequence induce antiviral genes selectively HPV-infected expressing protein.This study focuses on use an HPV-specific promoter comprised four drive IL-12, leading antitumor effect HPV-positive murine tumor model.The strategy was implemented via therapy using adenoviral vectors with recombinant IL-12 E2BS-IL-12.We demonstrate functional vitro vivo through transactivation transcription factor.