New cyclodepsipeptides, enniatins D, E and F produced by Fusarium sp. FO-1305.

• DOI: 10.7164/antibiotics.45.1207
• Publication Date: 2012-02-29T22:42:24Z
• Authors: HIROSHI TOMODA; HIROYUKI NISHIDA; XIN-HUI HUANG; ROKURO MASUMA; YOUNG KOOK KIM; SATOSHI OMURA
• Keywords: IC50

New cyclodepsipeptides named enniatins D, E and F were isolated from the culture broth of Fusarium sp. FO-1305 as inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT). The respective structures of enniatins D, E and F were determined to be cyclo[D-alpha-hydroxyisovaleryl(D-Hiv)-L-N-methylleucinyl(L- Me-Leu)-D-Hiv-L-N-methylvalinyl(L-Me-Val)-D-Hiv-L-Me-Val], a mixture of cyclo-[D-Hiv-L-Me-Leu-D-Hiv-L-N-methylisoleucinyl(L-Me-Ile)-D-Hiv- L-Me-Val] and cyclo(D-Hiv-L-Me-Ile-D-Hiv-L-Me-Leu-D-Hiv-L-Me-Val), and cyclo(D-Hiv-L-Me-Leu-D-Hiv-L-Me-Ile-D-Hiv-L-Me-Ile) by spectral analyses and chemical degradation. The IC50 values of enniatins D, E and F for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 87, 57 and 40 microM, respectively.