Heliquinomycin, a New Inhibitor of DNA Helicase, Produced by Streptomyces sp. MJ929-SF2. I. Taxonomy, Production, Isolation, Physico-chemical Properties and Biological Activities.

• DOI: 10.7164/antibiotics.49.752
• Publication Date: 2012-02-29T22:45:19Z
• Authors: MAKOTO CHINO; KIYOHIRO NISHIKAWA; MAYA UMEKITA; CHIGUSA HAYASHI; TAKAKO YAMAZAKI; TOSHIO TSUCHIDA; TSUTOMU SAWA; MASA HAMADA; TOMIO TAKEUCHI
• Keywords: HeLa; Sephadex; Streptomycetaceae

Heliquinomycin was isolated as a part of a program designed to find inhibitors of DNA helicase from microbial sources. It was purified from the culture broth of Streptomyces sp. MJ929-SF2 by solvent extraction and serial chromatographies of centrifugal partition chromatography, Sephadex LH-20 and Capcell Pak C18 (HPLC). The isolated red powder was analyzed to have the molecular formula of C33H30O17. It inhibited partially purified DNA helicase from HeLa cell in a non-competitive manner with the inhibition constant (Ki) of 6.8 mM. Heliquinomycin exhibited biological activity against microorganisms including MRSA, and cultured cell lines.