High-throughput Minitaturized RNA-Seq Library Preparation

• DOI: 10.7171/jbt.20-3104-004
• Publication Date: 2020-10-22T07:00:11Z
• Authors: Samuel Mildrum; Austin Hendricks; Alexei Stortchevoi; Noelani Kamelamela; Vincent L. Butty; Stuart S. Levine
• Keywords: Automation; 0303 health sciences; 03 medical and health sciences; High-Throughput Nucleotide Sequencing; RNA; Reproducibility of Results; Genomics; RNA-Seq; Gene Library; Workflow

Advances in next-generation sequencing technologies have allowed RNA sequencing to become an increasingly time efficient, cost-effective, and accessible tool for genomic research. We present here an automated and miniaturized workflow for RNA library preparation that minimizes reagent usage and processing time required per sample to generate Illumina compatible libraries for sequencing. The reduced-volume libraries show similar behavior to full-scale libraries with comparable numbers of genes detected and reproducible clustering of samples.