ANTICANCER POTENTIAL OF NOVEL PALLADIUM(II) COMPLEXES WITH ACYL PYRUVATES AS LIGANDS: DNA AND BSA INTERACTIONS AND MOLECULAR DOCKING STUDY

• DOI: 10.7251/sted2401001j
• Publication Date: 2025-03-10T23:27:46Z
• Authors: Nenad Joksimović; Jelena Petronijević; Marina Serafinović; Nenad Janković; Dejan Baskic
• Keywords: Docking (animal); Molecular model

Bearing in mind that some palladium complexes showed good antitumor potential while exhibiting less kidney toxicity comparing to cisplatin, discover a new agents for chemotherapy with improved properties two novel palladium(II) [Pd (L)2] (3A and 3B) acyl pyruvates (O,O bidentate ligands) were synthesized characterized by spectral (UV-Vis, IR, NMR, ESI-MS) elemental analysis. The analyzed their cytotoxic on human cancer cell lines (HeLa MDA-MB 231) normal fibroblasts (MRC-5). Results complex 3A displayed very activity, 3B had moderate activity the tested tumor lines. After 48h incubation 3A, his IC50 values similar of cisPt. Notably, all fetal lung (MRC-5) higher than 100 μM, indicating selectivity. In addition, induced apoptotic type death, cycle arrest G0/G1 phase both HeLa 231 we revealed can be useful as adjuvants therapy reducing dose cisplatin this manner its’ side effects. For investigations interactions between CT-DNA or bovine serum albumin (BSA) fluorometric titrations method was used. obtained results implied has great affinity displace ethidium bromide (EB) from EB-DNA through intercalation, suggesting strong competition EB. fluorescence titration BSA fluorescence quenching happens because formation 3A-BSA complex. Obtained Ka value is optimal range signifying appropriate amount transported distributed cells. order better understand binding newly DNA, molecular docking study further performed.