THE EFFECTS OF DIFFERENT STORING TEMPERATURES ON THE ACTIVITY OF SHEEP BLOOD AND PLASMA GLUTATHIONE PEROXIDASE

• DOI: 10.7251/vetjen1801038b
• Publication Date: 2018-06-15T03:29:33Z
• Authors: Dušan BOŠNJAKOVIĆ; Aleksandra PETROVIĆ; Olivera VALČIĆ; Ivan JOVANOVIĆ; Svetlana MILANOVIĆ
• Keywords: 0303 health sciences; 03 medical and health sciences

The aim of the study was to determine the stability of the activity ofglutathione peroxidases 1 and 3 during storage at +40C and -180C. Blood sampleswere taken from eight sheep and the activity of the enzyme was determined in theplasma (GPx3) and erythrocytes (GPx1) on the first, third, fifth and seventh day insamples stored at +40C and after one and three months in samples stored at -180C.GPx3 activity decreased significantly during storage at both temperatures, whileGPx1 remained steady even after three months of storage at -180C. Obtained resultsindicate that GPx3 activity has to be determined in fresh sheep plasma samples,while the activity of sheep erythrocyte GPx1 can be determined even after 3 monthsof storage at -180C.