Pumpkin powdery mildew disease severity influences the fungal diversity of the phyllosphere

• DOI: 10.7287/peerj.preprints.3278v1
• Publication Date: 2018-01-13T13:54:04Z
• Authors: Zhuo Zhang; Luyun Luo; Xinqiu Tan; Xiao Kong; Jianguo Yang; Duanhua Wang; Deyong Zhang; Decai Jin; Yong Liu
• Keywords: Phyllosphere; Blumeria graminis; Biotic component

Phyllosphere microbiota play a crucial role in plant-environment interactions and are influenced by biotic abiotic factors. However, there is little research on how pathogen s affect the microbial community. In this study, we collected 16 pumpkin ( Cucurbita moschata ) leaf samples showing symptoms of powdery mildew disease with different severity levels ranging from L1 (least severe) to L4 (most severe). We examined fungal community structure diversity Illumina MiSeq sequencing internal transcribed spacer (ITS) region ribosomal RNA genes. The communities were dominated members Basidiomycota Ascomycota. dominant genus was Podosphaera diseased leaves, which key responsible for mildew. Ascomycota increased abundance as L4, significantly more abundant than other microorganisms at P <0.05). richness L2, then declined L2 likely due pressure L4. Maintaining species phyllosphere will be an important part managing control agroecological system essential step toward predictable biocontrol pumpkin.