Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

• DOI: 10.7314/apjcp.2012.13.10.5131
• Publication Date: 2013-02-22T02:57:41Z
• Authors: Aied M. Alabsi; Rola Ali; Abdul Manaf Ali; Sami Abdo Radman ...; Hazlan Harun; Noor H. Abu Kasim; Abdulsamad Alsalahi
• Keywords: 0301 basic medicine; Plant Extracts; RK Dentistry; Blotting, Western; 610; Apoptosis; Breast Neoplasms; Fibroblasts; In Vitro Techniques; Flow Cytometry; Plant Roots; S Phase; 3. Good health; Mice; 03 medical and health sciences; Pyrones; Colonic Neoplasms; Animals; Humans; Female; Cells, Cultured; Cell Proliferation; HeLa Cells

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.