Senescence as A Consequence of Ginsenoside Rg1Response on K562 Human Leukemia Cell Line

• DOI: 10.7314/apjcp.2012.13.12.6191
• Publication Date: 2013-05-15T05:08:37Z
• Authors: Jun Liu; Shi-Zhong Cai; Yue Zhou; Xian-Ping Zhang; Dian-Feng Liu; Rong Jiang; Ya-Ping Wang
• Keywords: K562 cells; Senescence; MTT assay

Traditional chemotherapy strategies for human leukemia commonly use drugs based on cytotoxicity to eradicate cancer cells. One predicament is that substantial damage normal tissues likely occur in the course of standard treatments. Obviously, it urgent explore therapies can effectively eliminate malignant cells without affecting Our previous studies indicated ginsenoside Rg1 (Rg1), a major active pharmacological ingredient ginseng, could delay hematopoietic stem cell senescence. However, whether induce senescence still unclear.In current study, K562 were subjected exposure. The optimal drug concentration and duration with was obtained by MTT colorimetric test. Effects cycle analyzed using flow cytometry SA-β-Gal staining. Colony-forming ability measured colony-assay. Telomere lengths assessed Southern blotting expression senescence-associated proteins P21, P16 RB Western blotting. Ultrastructural morphology changes observed transmission electron microscopy.K562 demonstrated maximum proliferation inhibition rate an 20 μ molL-1 48h, exhibiting dramatic morphological alterations including enlarged flat cellular morphology, larger mitochondria increased number lysosomes. Senescence associated-β-galactosidase (SA-β-Gal) activity increased. also had decreased colony formation, shortened telomere length as well reduction proliferating potential arrestin G2/M phase after interaction. associated significantly up-regulated.Ginsenoside state cells, which p21-Rb p16-Rb pathways.