Hydrogen Peroxide Promotes Epithelial to Mesenchymal Transition and Stemness in Human Malignant Mesothelioma Cells

• DOI: 10.7314/apjcp.2013.14.6.3625
• Publication Date: 2013-11-26T05:54:15Z
• Authors: Myung-Chul Kim; Feng-Ji Cui; Yongbaek Kim
• Keywords: Homeodomain Proteins; Mesothelioma; 0301 basic medicine; Epithelial-Mesenchymal Transition; Lung Neoplasms; Blotting, Western; Mesothelioma, Malignant; Apoptosis; Hydrogen Peroxide; Nanog Homeobox Protein; Cadherins; Hypoxia-Inducible Factor 1, alpha Subunit; Oxidants; 3. Good health; Immunoenzyme Techniques; Oxidative Stress; 03 medical and health sciences; 0302 clinical medicine; Cell Movement; Cell Adhesion; Neoplastic Stem Cells; Humans; Octamer Transcription Factor-3; Cell Proliferation

Reactive oxygen species (ROS) are known to promote mesothelial carcinogenesis that is closely associated with asbestos fibers and inflammation. Epithelial mesenchymal cell transition (EMT) an important process involved in the progression of tumors, providing cancer cells aggressiveness. The present study was performed determine if EMT induced by H2O2 human malignant mesothelioma (HMM) cells. Cultured HMM were treated H2O2, followed measuring expression levels EMT-related genes proteins. Immunohistochemically, TWIST1 confined sarcomatous tissues, but not epithelioid Treatment promoted EMT, as indicated increased vimentin, SLUG TWIST1, decreased E-cadherin expression. Expression stemness such OCT4, SOX2 NANOG also significantly treatment H2O2. Alteration these mediated via activation hypoxia inducible factor 1 alpha (HIF-1α) transforming growth beta (TGF-β1). Considering results excess ROS, suggests oxidative stress may play a critical role promoting processes enhancing genes.