Hydrogen Peroxide Promotes Epithelial to Mesenchymal Transition and Stemness in Human Malignant Mesothelioma Cells
Homeodomain Proteins
Mesothelioma
0301 basic medicine
Epithelial-Mesenchymal Transition
Lung Neoplasms
Blotting, Western
Mesothelioma, Malignant
Apoptosis
Hydrogen Peroxide
Nanog Homeobox Protein
Cadherins
Hypoxia-Inducible Factor 1, alpha Subunit
Oxidants
3. Good health
Immunoenzyme Techniques
Oxidative Stress
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Adhesion
Neoplastic Stem Cells
Humans
Octamer Transcription Factor-3
Cell Proliferation
DOI:
10.7314/apjcp.2013.14.6.3625
Publication Date:
2013-11-26T05:54:15Z
AUTHORS (3)
ABSTRACT
Reactive oxygen species (ROS) are known to promote mesothelial carcinogenesis that is closely associated with asbestos fibers and inflammation. Epithelial mesenchymal cell transition (EMT) an important process involved in the progression of tumors, providing cancer cells aggressiveness. The present study was performed determine if EMT induced by H2O2 human malignant mesothelioma (HMM) cells. Cultured HMM were treated H2O2, followed measuring expression levels EMT-related genes proteins. Immunohistochemically, TWIST1 confined sarcomatous tissues, but not epithelioid Treatment promoted EMT, as indicated increased vimentin, SLUG TWIST1, decreased E-cadherin expression. Expression stemness such OCT4, SOX2 NANOG also significantly treatment H2O2. Alteration these mediated via activation hypoxia inducible factor 1 alpha (HIF-1α) transforming growth beta (TGF-β1). Considering results excess ROS, suggests oxidative stress may play a critical role promoting processes enhancing genes.
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