IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

Radioresistance Clonogenic assay
DOI: 10.7314/apjcp.2014.15.23.10115 Publication Date: 2015-02-23T21:42:04Z
ABSTRACT
The aim of this study was to investigate effects adipose-derived mesenchymal stem cells (AMSCs) on radioresistance breast cancer cells.MTT assays were used detect any influence AMSC supernatants proliferation cells; cell migration determine the effect recruitment AMSCs; survival fraction post-irradiation assessed by clonogenic assay; γ-H2AX foci number determined via fluorescence microscopy; and expression IGF-1R detected Western blotting.AMSC promoted cells. Breast could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for cells.Our results suggest that in tumor microenvironment may affect outcome radiotherapy vitro.
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