IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

• DOI: 10.7314/apjcp.2014.15.23.10115
• Publication Date: 2015-02-23T21:42:04Z
• Authors: Hui-Ying Yang; Rong-Mei Qu; Xiao-Shan Lin; Tong-Xin Liu; Quan-Quan Sun; Chun Yang; Xiao-Hong Li; Wei Lu; Xiao-Fang Hu; Jing-Xing Dai; Lin Yuan
• Keywords: Radiation; Breast Neoplasms; Mesenchymal Stem Cells; Receptors, Somatomedin; Receptor, IGF Type 1; 3. Good health; Histones; 03 medical and health sciences; 0302 clinical medicine; Adipose Tissue; Cell Movement; Cell Line, Tumor; MCF-7 Cells; Tumor Microenvironment; Humans; Female; Insulin-Like Growth Factor I; Cell Proliferation

The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells.MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; γ-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting.AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells.Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.