IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

• DOI: 10.7314/apjcp.2014.15.23.10115
• Publication Date: 2015-02-23T21:42:04Z
• Authors: Hui-Ying Yang; Rong-Mei Qu; Xiao-Shan Lin; Tong-Xin Liu; Quan-Quan Sun; Chun Yang; Xiao-Hong Li; Wei Lu; Xiao-Fang Hu; Jing-Xing Dai; Lin Yuan
• Keywords: Radioresistance; Clonogenic assay

The aim of this study was to investigate effects adipose-derived mesenchymal stem cells (AMSCs) on radioresistance breast cancer cells.MTT assays were used detect any influence AMSC supernatants proliferation cells; cell migration determine the effect recruitment AMSCs; survival fraction post-irradiation assessed by clonogenic assay; γ-H2AX foci number determined via fluorescence microscopy; and expression IGF-1R detected Western blotting.AMSC promoted cells. Breast could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for cells.Our results suggest that in tumor microenvironment may affect outcome radiotherapy vitro.