Knockdown of GCF2/LRRFIP1 by RNAi Causes Cell Growth Inhibition and Increased Apoptosis in Human Hepatoma HepG2 Cells

• DOI: 10.7314/apjcp.2014.15.6.2753
• Publication Date: 2014-07-10T03:44:48Z
• Authors: Jing-Ping Li; Nai-Xia Cao; Ri-Ting Jiang; Shao-Jian He; Tian-Ming Huang; Bo Wu; De-Feng Chen; Ping Ma; Li Chen; Su-Fang Zhou; Xiao-Xun Xie; Guo-Rong Luo
• Keywords: Viability assay

Background: GC-binding factor 2 (GCF2) is a transcriptional regulator that represses activity of the epidermal growth receptor (EGFR) by binding to specific GC-rich sequence in EGFR gene promoter. In addition this function, GCF2 has also been identified as tumor-associated antigen and regarded potentially valuable serum biomarker for early human hepatocellular carcinoma (HCC) diagnosis. high expressed most HCC tissues cell lines including HepG2. This study focused on influence proliferation apoptosis HepG2 cells. Materials Methods: expression at both mRNA protein levels cells was detected with reverse transcription (RT) PCR Western blotting, respectively. RNA interference (RNAi) technology used knock down expression. Afterwards, viability analyzed Cell Counting Kit-8 (CCK-8), caspase 3 flow cytometry Caspase Activity Kit, Results: Specific down-regulation caused inhibition, increased Conclusions: These primary results suggest may cells, provides molecular basis further investigation into possible mechanism HCC.