Macrophage-secreted Exosomes Delivering miRNA-21 Inhibitor can Regulate BGC-823 Cell Proliferation

• DOI: 10.7314/apjcp.2015.16.10.4203
• Publication Date: 2015-09-10T14:37:38Z
• Authors: Jian-Jun Wang; Ze-You Wang; Rui Chen; Jing Xiong; Yong-Liang Yao; Jian-Hong Wu; Guang-Xin Li
• Keywords: Exosome

Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased levels BGC-823, but exerted an opposite effect. transfected had less cellular toxicity host cells compared conventional transfection methods. The promoted the migration ability reduced apoptosis of These observations indicate acts a promoter by targeting PDCD4 gene preventing through inhibition expression. Furthermore, exosome -mediated delivery resulted functionally more efficient Similar approaches could be useful modification target biomolecules vitro vivo. findings contribute our understanding functions carrier for therapy cancer.