Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum.

• DOI: 10.7497/j.issn.2095-3941.2015.0010
• Publication Date: 2015-09-01
• Authors: Huajun Gao; Yajing Chen; Duo Zuo; Mingming Xiao; Ying Li; Hua Guo; Ning Zhang; Ruibing Chen
• Keywords: Quantitative Analysis

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity screening for early HCC diagnosis. This study employed quantitative proteomic strategy analyze differential expression glycoproteins between normal control samples.Lectin affinity chromatography (LAC) was used enrich from samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling 2D liquid (LC) separations were performed examine levels detected proteins Western blot three proteins.A total 2,280 protein groups identified in samples patients by using LC-MS/MS method. Up 36 up-regulated serum, whereas 19 down-regulated. Three glycoproteins, namely, fibrinogen gamma chain (FGG), FOS-like antigen 2 (FOSL2), α-1,6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase B (MGAT5B) validated blot. All these samples.A glycoproteomic method established proven useful determine potential novel HCC.