Effect of 5-aza-2'-deoxycytidine combined with trichostatin A on RPMI-8226 cell proliferation, apoptosis and DLC-1 gene expression.

• DOI: 10.7534/j.issn.1009-2137.2014.02.017
• Publication Date: 2014-04-01
• Authors: Jing Guo; Xianqi Feng; Shumin Nie; Zhan Su; Xue Shi; Zhongguang Cui; Ling Zhang; Shiguo Liu; Fanjun Meng; Chunting Zhao
• Keywords: Trichostatin A; Histone deacetylase inhibitor

This study was aimed to investigate the effects of DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-Aza-CdR) and histone deacetylase trichostatin A (TSA) on DLC-1 gene transcription regulation molecular biological behaviours in human multiple myeloma RPMI-8226 cells. The cells were treated respectively with 5-Aza-CdR TSA alone, or both combination; cell proliferation apoptosis, expression, protein expression Ras homolog family member (RhoA) Ras-related C3 botulinum toxin substrate 1 (Rac1) examined by CCK-8 method, RT-PCR ELISA, respectively. results showed that had growth inhibitory apoptosis-inducing dose-dependent manner (P < 0.05). Compared a single drug (5-Aza-CdR alone), significantly enhanced after treatment combination weakly expressed control group; alone its re-expression dose-dependently plus significantly.Compared control, decreased RhoA Rac1 It is concluded can effectively reverse cells; has synergistic effect re-expression. have significant These may be related inhibition Rho/Rho kinase signalling pathway.