effect of ikk2 inhibitor ly2409881 on diffuse large b cell lymphoma cell apoptosis and its mechanism

• DOI: 10.7534/j.issn.1009-2137.2018.04.024
• Publication Date: 2018-08-01
• Authors: Wei Sang; Xiang Gao; Pu Yang; Jun Jiao; Xiao-Kun Sun; Linyan Xu; Kailin Xu
• Keywords: Pyrimidines; Cell Line, Tumor; Humans; Apoptosis; Lymphoma, Large B-Cell, Diffuse; Thiophenes; Cell Proliferation; I-kappa B Kinase

To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G1 cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.LY2409881 inhibits the proliferation of DLBCL cell lines, causes G1 cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.