Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

• DOI: 10.7554/elife.02131
• Publication Date: 2014-04-08T18:03:31Z
• Authors: Sandra Richter; Marika Kientz; Sabine Brumm; Mads Eggert Nielsen; Misoon Park; Richard Gavidia; Cornelia Krause; Ute Voss; Hauke Beckmann; Ulrike Mayer; York-Dieter Stierhof; Gerd Jürgens
• Keywords: cell division; ARF-GEF; QH301-705.5; Arabidopsis Proteins; Science; Q; R; Arabidopsis; Golgi Apparatus; Cell Biology; recycling; post-Golgi trafficking; gegulation of vesicle traffic; Endocytosis; 12. Responsible consumption; secretion; Protein Transport; Medicine; Biology (General); Cell Division

Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators trafficking, mediating late secretion from trans-Golgi network not recycling endocytosed proteins plasma membrane, although TGN also functions an early endosome plants. contrast, BIG1-4 are absolutely required for both newly synthesized cell-division plane during counteracting membrane. This change secretory pathway mediated by ARF-GEFs confers specificity cargo delivery division might thus ensure completed on time absence cytokinesis-interphase checkpoint. DOI: http://dx.doi.org/10.7554/eLife.02131.001.