Re-visiting the trans insertion model for complexin clamping

• DOI: 10.7554/elife.04463
• Publication Date: 2015-04-02T15:35:03Z
• Authors: Shyam S Krishnakumar; Feng Li; Jeff Coleman; Curtis M Schauder; Daniel Kümmel; Frederic Pincet; James E Rothman; Karin M Reinisch
• Keywords: Isothermal Titration Calorimetry; Clamp

We have previously proposed that complexin cross-links multiple pre-fusion SNARE complexes via a trans interaction to function as clamp on SNARE-mediated neurotransmitter release. A recent NMR study was unable detect the clamping of and therefore questioned previous interpretation fluorescence resonance energy transfer isothermal titration calorimetry data which model originally based. Here we present new biochemical underscore validity our continued relevancy insertion for clamping.