A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila

• DOI: 10.7554/elife.05338
• Publication Date: 2015-04-01T14:14:10Z
• Authors: Sonal Nagarkar-Ja...; Pei-Tseng Lee; Megan E Campbell; Kuchuan Chen; Stephanie Anguian...; Manuel Cantu Guti...; Theodore Busby; Wen-Wen Lin; Yuchun He; Karen L Schulze; Benjamin W Booth; Martha Evans-Holm; Koen JT Venken; Robert W Levis; Allan C Spradling; Roger A Hoskins; Hugo J Bellen
• Keywords: Coding region

Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions which 2854 are inserted in coding introns. They allowed us to create library 400 GFP-tagged genes. We show that 72% internally tagged proteins functional, and more than 90% can be imaged unfixed tissues. Moreover, the mRNAs knocked down by RNAi against GFP (iGFPi), efficiently deGradFP technology. The phenotypes associated with RNA protein knockdown typically correspond severe loss function or null mutant phenotypes. Finally, demonstrate reversible, spatial, temporal larvae adult flies. This new strategy strains allows unprecedented vivo manipulations flies for many These strategies will likely extend vertebrates.