Glycosylphosphatidylinositol-anchored proteins as chaperones and co-receptors for FERONIA receptor kinase signaling in Arabidopsis

• DOI: 10.7554/elife.06587
• Publication Date: 2015-06-08T10:34:46Z
• Authors: Chao Li; Fang-Ling Yeh; Alice Y Cheung; Qiaohong Duan; Daniel Kita; Ming-Che Liu; Jacob Maman; Emily J Luu; Brendan W Wu; Laura Gates; Methun Jalal; Amy Kwong; Hunter Carpenter; Hen-Ming Wu
• Keywords: Chaperone (clinical); ER retention

The Arabidopsis receptor kinase FERONIA (FER) is a multifunctional regulator for plant growth and reproduction. Here we report that the female gametophyte-expressed glycosylphosphatidylinositol-anchored protein (GPI-AP) LORELEI seedling-expressed LRE-like GPI-AP1 (LLG1) bind to extracellular juxtamembrane region of FER show this interaction pivotal function. LLG1 interacts with in endoplasmic reticulum on cell surface, loss function induces cytoplasmic retention FER, consistent transport from plasma membrane complex LLG1. We further demonstrate component FER-regulated RHO GTPase signaling fer llg1 mutants display indistinguishable growth, developmental phenotypes, analogous how lre share similar reproductive defects. Together our results support LLG1/LRE acting as chaperone co-receptor elucidate mechanism by which GPI-APs enable capacity surface receptor.