Exploiting functional regions in the viral RNA genome as druggable entities

• DOI: 10.7554/elife.103923.2
• Publication Date: 2025-05-19T14:30:54Z
• Authors: Dehua Luo; Yingge Zheng; Zhiyuan Huang; Zi Wen; Lijun Guo; Yingxiang Deng; Qingling Li; Yuqing Bai; Shozeb Haider; Dengguo Wei

Abstract RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means for controlling viral infections. An essential prerequisite to their design depends on identifying conserved and functional viral RNA structures in cells. Techniques that probe RNA structures in situ have been developed recently including SHAPE-MaP, which has been helpful in analyzing the secondary structures of RNA. In this study, we report on the application of SHAPE-MaP to the Porcine Epidemic Diarrhoea Virus (PEDV) RNA genome to categorize different functional regions including potential quadruplex forming sequence and target sites of siRNA. Our results show that these structures can be exploited to inhibit viral proliferation and that SHAPE-MaP is an effective method to the identification of secondary structures in RNA genomes.