Exploiting functional regions in the viral RNA genome as druggable entities

• DOI: 10.7554/elife.103923.2
• Publication Date: 2025-05-19T14:30:54Z
• Authors: Dehua Luo; Yingge Zheng; Zhiyuan Huang; Zi Wen; Lijun Guo; Yingxiang Deng; Qingling Li; Yuqing Bai; Shozeb Haider; Dengguo Wei
• Keywords: Druggability

Abstract RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means for controlling viral infections. An essential prerequisite to their design depends on identifying conserved and functional RNA structures in cells. Techniques that probe situ have been developed recently including SHAPE-MaP, which has helpful analyzing the secondary of RNA. In this study, we report application SHAPE-MaP Porcine Epidemic Diarrhoea Virus (PEDV) genome categorize different regions potential quadruplex forming sequence target sites siRNA. Our results show these can be exploited inhibit proliferation is an effective method identification genomes.