Mechanisms of PP2A-Ankle2 dependent nuclear reassembly after mitosis

• DOI: 10.7554/elife.104233.1
• Publication Date: 2024-11-27T16:26:01Z
• Authors: Jingjing Li; Xinyue Wang; Laia Jordana; Éric Bonneil; Victoria Ginestet; Momina Ahmed; Mohammed Bourouh; Cristina Mirela P...; T Martin Schmeing; Pierre Thibault; Vincent Archambault
• Keywords: mitosis; QH301-705.5; Science; nucleus; Q; R; Mitosis; nuclear envelope; PP2A; Medicine; ankle2; BAF; Biology (General); Cancer

In animals, mitosis involves the breakdown of nucleus. The reassembly a nucleus after requires reformation nuclear envelope around single mass chromosomes. This process Ankle2 which interacts with PP2A and promotes function Barrier-to-Autointegration Factor (BAF). Upon dephosphorylation, BAF dimers cross-bridge chromosomes bind lamins transmembrane proteins reassembling envelope. How functions in is incompletely understood. Using combination approaches Drosophila , along structural modeling, we show for first time that regulatory subunit PP2A, explaining how it dephosphorylation. addition, discovered endoplasmic reticulum protein Vap33, required localization at during telophase. We identified interaction sites Vap33 on Ankle2. Through genetic rescue experiments, Ankle2/PP2A essential Ankle2/Vap33 also this process. Our study sheds light molecular mechanisms post-mitotic suggests not merely source membranes process, but provides localized enzymatic activity.