Multi-omics investigation of spontaneous T2DM macaque reveals gut microbiota promote T2DM by up-regulating the absorption of excess palmitic acid

• DOI: 10.7554/elife.104355.1
• Publication Date: 2025-02-07T08:27:14Z
• Authors: Xu Liu; Shengzhi Yang; Yuchen Xie; Cong Jiang; Ke Shang; Jinxia Luo; Lin Zhang; Gang Hu; Qinghua Liu; Bisong Yue; Zhenxin Fan; Zhanlong He; Jing Li
• Keywords: Metabolome

Although gut microbiota and lipid metabolites have been suggested to be closely associated with type 2 diabetes mellitus (T2DM), the interactions between microbiota, host in T2DM development remains unclear. Rhesus macaques may best animal model investigate these relationships given their spontaneous of T2DM. We identified eight conducted a comprehensive study investigating using multi-omics sequencing technology. Our results from 16S rRNA, metagenome, metabolome transcriptome analyses that imbalance, tryptophan metabolism fatty acid β oxidation disorders, long-chain (LCFA) accumulation, inflammation occurred macaques. verified accumulation palmitic (PA) activation Importantly, mice transplanted macaque fecal fed high PA diet developed prediabetes within 120 days. determined mediated absorption excess ileum, resulting serum consequently leading mice. In particular, we demonstrated specific composition was probably involved process. This provides new insight into confirms causative effect on development.