The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods

• DOI: 10.7554/elife.105737.1
• Publication Date: 2025-04-02T16:23:26Z
• Authors: Ziv Yaniv; Ifeanyichukwu U A...; Leanne Arakkal; Armando J Arroyo-...; Rebecca T Beuschel; Katy Börner; Colin J Chu; Beatrice Clark; Menna R Clatworthy; Jake Colautti; Fabian Coscia; Joshua Croteau; Saven Denha; Rose Dever; Walderez O Dutra; Sonja Fritzsche; Spencer Fullam; Michael Y Gerner; Anita Gola; Kenneth J Gollob; Jonathan M Hernandez; Jyh Liang Hor; Hiroshi Ichise; Zhixin Jing; Danny Jonigk; Evelyn Kandov; Wolfgang Kastenmü...; Joshua FE Koenig; Aanandita Kothurkar; Rosa K Kortekaas; Alexandra Y Kreins; Ian T Lamborn; Yuri Lin; Katia Luciano Per...; Aleksandra Lunich; Jean CS Luz; Ryan B MacDonald; Chen Makranz; Vivien I Maltez; John E McDonough; Ryan V Moriarty; Juan M Ocampo-God...; Vitoria M Olyntho; Annette Oxenius; Kartika Padhan; Kirsten Remmert; Nathan Richoz; Edward C Schrom; Wanjing Shang; Lihong Shi; Rochelle M Shih; Emily Speranza; Salome Stierli; Sarah A Teichmann; Tibor Z Veres; Megan Vierhout; Brianna T Wachter; Margaret Williams; Nathan Zangger; Ronald N Germain; Andrea J Radtke
• Keywords: Base (topology); Imaging technology

The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D 3D immunofluorescence imaging methods. design of the modeled after efforts in open-source software community includes three facets: development platform (GitHub), static website, service data archiving. facilitates practice open science throughout research life cycle by providing validation recommended non-recommended reagents, e.g., primary secondary antibodies. In addition to reporting negative data, empowers method adoption evolution venue sharing protocols, videos, datasets, software, publications. A dedicated discussion forum fosters sense among while addressing questions not covered published manuscripts. Together, scientists from around world are advancing scientific discovery at faster pace, reducing wasted time effort, instilling greater confidence resulting data.The data.