Interdigitating Modules for Visual Processing During Locomotion and Rest in Mouse V1

• DOI: 10.7554/elife.106468.1
• Publication Date: 2025-04-16T14:30:33Z
• Authors: Andrew M Meier; Rinaldo D D’Souza; Weiqing Ji; Edward B Han; Andreas Burkhalter
• Keywords: Rest (music); Visual processing

Layer 1 of V1 has been shown to receive locomotion-related signals from the dorsal lateral geniculate (dLGN) and posterior (LP) thalamic nuclei (Roth et al., 2016). Inputs dLGN terminate in M2+ patches while inputs LP target M2− interpatches (D’Souza 2019) suggesting that motion related are processed distinct networks. Here, we investigated by calcium imaging head-fixed awake mice whether L2/3 neurons underneath L1 modules differentially activated locomotion, networks feedback connections higher cortical areas may contribute these differences. We found strongly locomotion-modulated cell clusters during visual stimulation were aligned with interpatches, weakly modulated cells clustered under patches. Unlike patch cells, pairs interpatch showed increased correlated variability transients when sites visuotopic map far apart, activity is integrated across large parts field. Pathway tracing further suggests strong locomotion modulation relies on looped, like-to-like between apical dendrites MOs-, PM- RSP-projecting input L1. SST neurons, interneurons influence firing specific subnetworks controlling excitability interpatches.