Cell type-specific long-range connections of basal forebrain circuit

• DOI: 10.7554/elife.13214
• Publication Date: 2016-09-19T11:00:09Z
• Authors: Johnny Phong Do; Min Xu; Seung-Hee Lee; Wei-Cheng Chang; Siyu Zhang; Shinjae Chung; Tyler J Yung; Jiang Lan Fan; Kazunari Miyamichi; Liqun Luo; Yang Dan
• Keywords: Forebrain

The basal forebrain (BF) plays key roles in multiple brain functions, including sleep-wake regulation, attention, and learning/memory, but the long-range connections mediating these functions remain poorly characterized. Here we performed whole-brain mapping of both inputs outputs four BF cell types - cholinergic, glutamatergic, parvalbumin-positive (PV+) somatostatin-positive (SOM+) GABAergic neurons mouse brain. Using rabies virus -mediated monosynaptic retrograde tracing to label adeno-associated trace axonal projections, identified numerous areas connected BF. different were qualitatively similar, output projections showed marked differences. glutamatergic SOM+ strongly reciprocal, while those cholinergic PV+ more unidirectional. These results reveal wiring diagram circuit with highly convergent divergent point functional commonality specialization types.