Regulation of X-linked gene expression during early mouse development by Rlim

• DOI: 10.7554/elife.19127
• Publication Date: 2016-09-19T12:00:13Z
• Authors: Feng Wang; JongDae Shin; Jeremy M Shea; Jun Yu; Ana Bošković; Meg Byron; Xiaochun Zhu; Alex K Shalek; Aviv Regev; Jeanne B Lawrence; Eduardo M Torres; Lihua J Zhu; Oliver J Rando; Ingolf Bach
• Keywords: XIST

Mammalian X-linked gene expression is highly regulated as female cells contain two and male one X chromosome (X). To adjust the dosage between genders, mouse preimplantation embryos undergo an imprinted form of inactivation (iXCI) that requires both Rlim (also known Rnf12) long non-coding RNA Xist. Moreover, it thought from single active upregulated to correct for bi-allelic autosomal (A) expression. We have combined genetics with RNA-seq on investigate functions temporal regulation iXCI Our results reveal crucial roles maintenance high Xist levels, clouds X-silencing in at blastocyst stages, while initial appears Rlim-independent. find further X/A upregulation initiated early embryos.