ICE1 promotes the link between splicing and nonsense-mediated mRNA decay

• DOI: 10.7554/elife.33178
• Publication Date: 2018-03-12T13:00:15Z
• Authors: Thomas D Baird; Ken Chih-Chien Cheng; Yu-Chi Chen; Eugen Buehler; Scott E Martin; James Inglese; J Robert Hogg
• Keywords: Nonsense-Mediated Decay; Genetic screen

The nonsense-mediated mRNA decay (NMD) pathway detects aberrant transcripts containing premature termination codons (PTCs) and regulates expression of 5–10% non-aberrant human mRNAs. To date, most proteins involved in NMD have been identified by genetic screens model organisms; however, the increased complexity gene regulation cells suggests that additional may participate pathway. identify required for NMD, we performed a genome-wide RNAi screen against >21,000 genes. Canonical members were highly enriched as top hits siRNA screen, along with numerous candidate factors, including conserved ICE1/KIAA0947 protein. RNAseq studies reveal depletion ICE1 globally enhances accumulation stability NMD-target Further, our data suggest uses putative MIF4G domain to interact exon junction complex (EJC) promotes association protein UPF3B EJC.