The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution

• DOI: 10.7554/elife.34085
• Publication Date: 2018-08-15T00:00:16Z
• Authors: Michael Luke Carlson; John William Young; Zhiyu Zhao; Lucien Fabre; Daniel Jun; Jianing Li; Jun Li; Harveer Singh Dhupar; Irvin Wason; Allan T Mills; J Thomas Beatty; John S Klassen; Isabelle Rouiller; Franck Duong
• Keywords: Nanodisc; Structural Biology

Membrane proteins are difficult to work with due their insolubility in aqueous solution and quite often poor stability detergent micelles. Here, we present the peptidisc for facile capture into water-soluble particles. Unlike nanodisc, which requires scaffold of different lengths precise amounts matching lipids, reconstitution solubilized only a short amphipathic bi-helical peptide (NSPr) no extra lipids. Multiple copies wrap around shield membrane-exposed part target protein. We demonstrate effectiveness this ‘one size fits all’ method using five membrane protein assemblies (MalFGK2, FhuA, SecYEG, OmpF, BRC) during ‘on-column’, ‘in-gel’, ‘on-bead’ embedded within purification protocol. The is rapid cost-effective, it may emerge as universal tool high-throughput stabilization advance modern biological studies.