OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development
5-Hydroxymethylcytosine
Epigenome
DOI:
10.7554/elife.34870
Publication Date:
2018-10-16T12:01:30Z
AUTHORS (18)
ABSTRACT
TET enzymes convert 5-methylcytosine to 5-hydroxymethylcytosine and higher oxidized derivatives. TETs stably associate with are post-translationally modified by the nutrient-sensing enzyme OGT, suggesting a connection between metabolism epigenome. Here, we show for first time that modification OGT enhances TET1 activity in vitro. We identify domain is necessary sufficient binding report point mutation disrupts TET1-OGT interaction. this interaction rescue hematopoetic stem cell production tet mutant zebrafish embryos, promotes TET1’s function during development. Finally, disrupting mouse embryonic cells changes abundance of TET2 5-methylcytosine, which accompanied alterations gene expression. These results link epigenetic control, may be relevant developmental disease processes regulated these two enzymes.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (55)
CITATIONS (50)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....