Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity

• DOI: 10.7554/elife.34961
• Publication Date: 2018-07-10T12:05:16Z
• Authors: Brogan Yarzabek; Anita J Zaitouna; Eli Olson; Gayathri N Silva; Jie Geng; Aviva Geretz; Rasmi Thomas; Sujatha Krishnakumar; Daniel S Ramon; Malini Raghavan
• Keywords: Antigen processing

The highly polymorphic human leukocyte antigen (HLA) class I molecules present peptide antigens to CD8+ T cells, inducing immunity against infections and cancers. Quality control mediated by loading complex (PLC) components is expected ensure the cell surface expression of stable peptide-HLA complexes. This exemplified HLA-B*08:01 in primary lymphocytes, with both level half-life at high end measured HLA-B stability hierarchies. Conversely, low on lymphocytes for three allotypes that bind peptides proline position 2, which are disfavored transporter associated processing. Surprisingly, these lymphocyte-specific differences become reversed or altered monocytes, display larger intracellular pools HLA than lymphocytes. Together, findings indicate allele cell-dependent variations acquisition pathways influence levels, half-lives receptivity exogenous antigens.