Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells

• DOI: 10.7554/elife.36131
• Publication Date: 2018-06-15T12:00:15Z
• Authors: Sicong He; Jiahao Chen; Yunyun Jiang; Yi Wu; Lu Zhu; Wan Jin; Changlong Zhao; Tao Yu; Tienan Wang; Shuting Wu; Xi Lin; Jianan Y Qu; Zilong Wen; Wenqing Zhang; Jin Xu
• Keywords: Dorsal aorta; Fate mapping; Developmental Biology; Epidermis (zoology)

The origin of Langerhans cells (LCs), which are skin epidermis-resident macrophages, remains unclear. Current lineage tracing LCs largely relies on the promoter-Cre-LoxP system, often gives rise to contradictory conclusions with different promoters. Thus, reinvestigation an improved method is necessary. Here, using a laser-mediated temporal-spatial resolved cell labeling method, we demonstrated that most adult originated from ventral wall dorsal aorta (VDA), equivalent mouse aorta, gonads, and mesonephros (AGM), where both hematopoietic stem (HSCs) non-HSC progenitors generated. Further fine-fate mapping analysis revealed appearance in zebrafish was correlated development HSCs, but not T progenitors. Finally, showed tissue-resident macrophages brain, liver, heart, gut also HSCs. results our study challenged EMP-origin theory for LCs.