Proteolytic maturation of α2δ controls the probability of synaptic vesicular release

• DOI: 10.7554/elife.37507
• Publication Date: 2018-06-19T12:00:45Z
• Authors: Laurent Ferron; Ivan Kadurin; Annette C Dolphin
• Keywords: Cleavage (geology); Proteolytic enzymes

Auxiliary α2δ subunits are important proteins for trafficking of voltage-gated calcium channels (CaV) at the active zones synapses. We have previously shown that post-translational proteolytic cleavage is essential their modulatory effects on N-type (CaV2.2) (Kadurin et al., 2016). extend these results here by showing probability presynaptic vesicular release reduced when an uncleaved expressed in rat neurons and this inhibitory effect reversed restored. also show asynchronous influenced maturation α2δ−1, highlighting role CaV component release. present additional evidence CaV2.2 co-immunoprecipitates preferentially with cleaved wild-type α2δ. Our data indicate increases association α2δ−1 channel complex its function synaptic