An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila

• DOI: 10.7554/elife.38709
• Publication Date: 2018-08-09T12:01:35Z
• Authors: David Li-Kroeger; Oguz Kanca; Pei-Tseng Lee; Sierra Cowan; Michael T Lee; Manish Jaiswal; Jose Luis Salazar; Yuchun He; Zhongyuan Zuo; Hugo J Bellen
• Keywords: 0303 health sciences; Integrases; T2A-GAL4; gene editing; QH301-705.5; Science; Q; R; Genetics and Genomics; Mutagenesis, Insertional; 03 medical and health sciences; protein tagging; Trojan exons; Genes, Reporter; Gene Targeting; Medicine; Animals; swappable insertion cassette; Clustered Regularly Interspaced Short Palindromic Repeats; Drosophila; Biology (General); Entomology; Double Header

We generated two new genetic tools to efficiently tag genes in