HIPPO signaling resolves embryonic cell fate conflicts during establishment of pluripotency in vivo

• DOI: 10.7554/elife.42298
• Publication Date: 2018-12-11T00:01:31Z
• Authors: Tristan Frum; Tayler M Murphy; Amy Ralston
• Keywords: YAP1; Hippo signaling pathway; Cell fate determination; Inner cell mass

During mammalian development, the challenge for embryo is to override intrinsic cellular plasticity drive cells distinct fates. Here, we unveil novel roles HIPPO signaling pathway in controlling cell positioning and expression of Sox2, first marker pluripotency mouse early embryo. We show that maternal zygotic YAP1 WWTR1 repress Sox2 while promoting trophectoderm gene Cdx2 parallel. Yet, more sensitive than Yap1/Wwtr1 dosage, leading a state conflicted fate when YAP1/WWTR1 activity moderate. Remarkably, resolves by repositioning interior embryo, independent its role regulating expression. Rather, antagonizes apical localization Par complex components PARD6B aPKC. Thus, negative feedback between ensure robust lineage segregation.