Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID

• DOI: 10.7554/elife.47864
• Publication Date: 2019-09-19T12:00:12Z
• Authors: Andrea Mair; Shou-Ling Xu; Tess C Branon; Alice Y Ting; Dominique C Bergmann
• Keywords: Proteome

Defining specific protein interactions and spatially or temporally restricted local proteomes improves our understanding of all cellular processes, but obtaining such data is challenging, especially for rare proteins, cell types, events. Proximity labeling enables discovery neighborhoods defining functional complexes and/or organellar compositions. Recent technological improvements, namely two highly active biotin ligase variants (TurboID miniTurbo), allowed us to address challenging questions in plants: (1) what are vivo partners a low abundant key developmental transcription factor (2) the nuclear proteome type? Proteins identified with FAMA-TurboID include known interactors this stomatal novel proteins that could facilitate its activator repressor functions. Directing TurboID nuclei enabled purification type- subcellular compartment-specific proteins. Broad tests miniTurbo Arabidopsis Nicotiana benthamiana versatile vectors enable customization by plant researchers.