Single-cell transcriptomic evidence for dense intracortical neuropeptide networks

• DOI: 10.7554/elife.47889
• Publication Date: 2019-11-11T13:00:25Z
• Authors: Stephen J Smith; Uygar Sümbül; Lucas T Graybuck; Forrest Collman; Sharmishtaa Sesha...; Rohan Gala; Olga Gliko; Leila Elabbady; Jeremy A Miller; Trygve E Bakken; Jean Rossier; Zizhen Yao; Ed Lein; Hongkui Zeng; Bosiljka Tasic; Michael Hawrylycz
• Keywords: QH301-705.5; Science; Neocortex; Receptors, G-Protein-Coupled; transcriptomics; Mice; neocortex; Animals; Homeostasis; Gene Regulatory Networks; Biology (General); Protein Precursors; Visual Cortex; Neurons; Neuronal Plasticity; Gene Expression Profiling; Q; Neuropeptides; neuropeptides; neuron types; R; neuromodulation; Medicine; synaptic networks; Single-Cell Analysis; Neuroscience; Signal Transduction

Seeking new insights into the homeostasis, modulation and plasticity of cortical synaptic networks, we have analyzed results from a single-cell RNA-seq study 22,439 mouse neocortical neurons. Our analysis exposes transcriptomic evidence for dozens molecularly distinct neuropeptidergic modulatory networks that directly interconnect all This begins with discovery transcripts one or more neuropeptide precursor (NPP) neuropeptide-selective G-protein-coupled receptor (NP-GPCR) genes are highly abundant in all, very nearly Individual neurons express diverse subsets NP signaling palettes encoding 18 NPPs 29 NP-GPCRs. These 47 comprise 37 cognate NPP/NP-GPCR pairs, implying likelihood local signaling. Here, use neuron-type-specific patterns gene expression to offer specific, testable predictions regarding peptidergic neuromodulatory may play prominent roles homeostasis plasticity.