A regulatory microRNA network controls endothelial cell phenotypic switch during sprouting angiogenesis

• DOI: 10.7554/elife.48095
• Publication Date: 2020-01-24T11:00:18Z
• Authors: Stefania Rosano; Davide Corà; Sushant Parab; Serena Zaffuto; Claudio Isella; Roberta Porporato; Roxana Maria Hoza; Raffaele A Calogero; Chiara Riganti; Federico Bussolino; Alessio Noghero
• Keywords: Gene regulatory network

Angiogenesis requires the temporal coordination of proliferation and migration endothelial cells. Here, we investigated regulatory role microRNAs (miRNAs) in harmonizing angiogenesis processes a three-dimensional vitro model. We described microRNA network which contributes to observed down- upregulation proliferative migratory genes, respectively. Global analysis miRNA–target gene interactions identified two sub-network modules, first organized upregulated miRNAs connected with downregulated target genes second opposite features. miR-424–5p miR-29a-3p were selected for validation. Gain- loss-of-function approaches targeting these impaired angiogenesis, suggesting that modules are instrumental proliferation. Interestingly, its targets belong selective biomarker is able identify colorectal cancer patients who responding anti-angiogenic treatments. Our results provide view higher-order has potential diagnostic therapeutic insights.