Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation

• DOI: 10.7554/elife.52990
• Publication Date: 2020-01-06T11:00:14Z
• Authors: Harrison Tudor Evans; Liviu-Gabriel Bodea; Jürgen Götz
• Keywords: Proteome

The formation of spatial long-term memory (LTM) requires the de novo synthesis distinct sets proteins; however, a non-biased examination proteome in this process is lacking. Here, we generated novel mouse strain, which enables cell-type-specific labelling newly synthesised proteins with non-canonical amino acids (NCAAs) by genetically restricting expression mutant tRNA synthetase, NLL-MetRS, to hippocampal neurons. By combining technique an accelerated version active place avoidance task and bio-orthogonal acid tagging (BONCAT) followed SWATH quantitative mass spectrometry, identified 156 that were altered neurons during formation. In addition observing increased known important memory-related processes, such as glutamate receptor recycling, also associated mRNA splicing potential mechanism involved LTM