Translational control of one-carbon metabolism underpins ribosomal protein phenotypes in cell division and longevity

• DOI: 10.7554/elife.53127
• Publication Date: 2020-05-20T12:01:06Z
• Authors: Nairita Maitra; Chong He; Heidi M Blank; Mitsuhiro Tsuchiya; Birgit Schilling; Matt Kaeberlein; Rodolfo Aramayo; Brian K Kennedy; Michael Polymenis
• Keywords: Ribosomal protein; Metabolic pathway

A long-standing problem is how cells that lack one of the highly similar ribosomal proteins (RPs) often display distinct phenotypes. Yeast and other organisms live longer when they specific proteins, especially large 60S subunit ribosome. However, longevity neither associated with generation time RP deletion mutants nor bulk inhibition protein synthesis. Here, we queried actively dividing through cell cycle. Our data link transcriptional, translational, metabolic changes to phenotypes loss paralogous RPs. We uncovered translational control transcripts encoding enzymes methionine serine metabolism, which are part one-carbon (1C) pathways. Cells lacking Rpl22Ap, long-lived, have lower levels metabolites 1C metabolism. Loss increased wild type cells. pathways exist in all targeting relevant could represent interventions.