Microglial calcium signaling is attuned to neuronal activity in awake mice
Male
Neurons
0301 basic medicine
status epilepticus
0303 health sciences
Kainic Acid
Isoflurane
QH301-705.5
Science
Q
R
GCaMP
3. Good health
Mice
03 medical and health sciences
Status Epilepticus
DREADD
Medicine
Animals
Calcium
Female
Calcium Signaling
Microglia
Biology (General)
Neuroscience
DOI:
10.7554/elife.56502
Publication Date:
2020-07-27T12:00:26Z
AUTHORS (6)
ABSTRACT
Microglial calcium signaling underlies a number of key physiological and pathological processes in situ, but has not been studied in vivo in awake mice. Using multiple GCaMP6 variants targeted to microglia, we assessed how microglial calcium signaling responds to alterations in neuronal activity across a wide range. We find that only a small subset of microglial somata and processes exhibited spontaneous calcium transients in a chronic window preparation. However, hyperactive shifts in neuronal activity (kainate status epilepticus and CaMKIIa Gq DREADD activation) triggered increased microglial process calcium signaling, often concomitant with process extension. Additionally, hypoactive shifts in neuronal activity (isoflurane anesthesia and CaMKIIa Gi DREADD activation) also increased microglial process calcium signaling. Under hypoactive neuronal conditions, microglia also exhibited process extension and outgrowth with greater calcium signaling. Our work reveals that microglia have highly distinct microdomain signaling, and that processes specifically respond to bi-directional shifts in neuronal activity through increased calcium signaling.
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