Microglial calcium signaling is attuned to neuronal activity in awake mice

• DOI: 10.7554/elife.56502
• Publication Date: 2020-07-27T12:00:26Z
• Authors: Anthony D Umpierre; Lauren L Bystrom; Yanlu Ying; Yong U Liu; Gregory Worrell; Long-Jun Wu
• Keywords: Premovement neuronal activity; Calcium Signaling; Calcium imaging

Microglial calcium signaling underlies a number of key physiological and pathological processes in situ, but has not been studied vivo awake mice. Using multiple GCaMP6 variants targeted to microglia, we assessed how microglial responds alterations neuronal activity across wide range. We find that only small subset somata exhibited spontaneous transients chronic window preparation. However, hyperactive shifts (kainate status epilepticus CaMKIIa Gq DREADD activation) triggered increased process signaling, often concomitant with extension. Additionally, hypoactive (isoflurane anesthesia Gi also signaling. Under conditions, microglia extension outgrowth greater Our work reveals have highly distinct microdomain specifically respond bi-directional through