Three-dimensional synaptic organization of the human hippocampal CA1 field

Adult Male 0301 basic medicine Medicina microanatomy QH301-705.5 Science Molecular Conformation 03 medical and health sciences autopsy 0302 clinical medicine synapse Cadaver Humans human Biology (General) CA1 Region, Hippocampal Aged Aged, 80 and over Q R dementia, electron microscopy, FIB/SEM, hippocampus, synapses Middle Aged 16. Peace & justice FIB/SEM microscopy 3. Good health Microscopy, Electron, Scanning Medicine Female Autopsy Neuroscience
DOI: 10.7554/elife.57013 Publication Date: 2020-07-21T00:00:34Z
ABSTRACT
The hippocampal CA1 field integrates a wide variety of subcortical and cortical inputs, but its synaptic organization in humans is still unknown due to the difficulties involved studying the human brain via electron microscope techniques. However, we have shown that the 3D reconstruction method using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) can be applied to study in detail the synaptic organization of the human brain obtained from autopsies, yielding excellent results. Using this technology, 24,752 synapses were fully reconstructed in CA1, revealing that most of them were excitatory, targeting dendritic spines and displaying a macular shape, regardless of the layer examined. However, remarkable differences were observed between layers. These data constitute the first extensive description of the synaptic organization of the neuropil of the human CA1 region.
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