Three-dimensional synaptic organization of the human hippocampal CA1 field
Adult
Male
0301 basic medicine
Medicina
microanatomy
QH301-705.5
Science
Molecular Conformation
03 medical and health sciences
autopsy
0302 clinical medicine
synapse
Cadaver
Humans
human
Biology (General)
CA1 Region, Hippocampal
Aged
Aged, 80 and over
Q
R
dementia, electron microscopy, FIB/SEM, hippocampus, synapses
Middle Aged
16. Peace & justice
FIB/SEM microscopy
3. Good health
Microscopy, Electron, Scanning
Medicine
Female
Autopsy
Neuroscience
DOI:
10.7554/elife.57013
Publication Date:
2020-07-21T00:00:34Z
AUTHORS (6)
ABSTRACT
The hippocampal CA1 field integrates a wide variety of subcortical and cortical inputs, but its synaptic organization in humans is still unknown due to the difficulties involved studying the human brain via electron microscope techniques. However, we have shown that the 3D reconstruction method using Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) can be applied to study in detail the synaptic organization of the human brain obtained from autopsies, yielding excellent results. Using this technology, 24,752 synapses were fully reconstructed in CA1, revealing that most of them were excitatory, targeting dendritic spines and displaying a macular shape, regardless of the layer examined. However, remarkable differences were observed between layers. These data constitute the first extensive description of the synaptic organization of the neuropil of the human CA1 region.
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