Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules

• DOI: 10.7554/elife.60833
• Publication Date: 2021-07-05T14:00:47Z
• Authors: Patricia Giselle ...; Olivia Bay; John Zinno; Michelle Gutwein; Hin Hark Gan; Vinay K Mayya; George Chung; Jia-Xuan Chen; Hala Fahs; Yu Guan; Thomas F Duchaine; Matthias Selbach; Fabio Piano; Kristin C Gunsalus
• Keywords: Granule (geology)

We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P condensation, form granules independently of in postembryonic line, balance each other regulating growth localization. MIP-2 contain two LOTUS domains regions homo- heterodimers. They bind anchor Vasa homolog GLH-1 within are jointly required for coalescence MEG-3, GLH-1, PGL proteins. Animals lacking show temperature-sensitive embryonic lethality, sterility, mortal lines. Germline phenotypes include defects stem cell self-renewal, meiotic progression, gamete differentiation. propose these serve as scaffolds organizing centers ribonucleoprotein networks help recruit essential RNA processing machinery to regulate key developmental transitions line.