AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

• DOI: 10.7554/elife.61669
• Publication Date: 2020-12-09T16:04:17Z
• Authors: Silvia Vergarajau...; Robert Becker; Ulrike Steffen; Maria Sharkova; Tilman Esser; Jana Petzold; Florian Billing; Michael S Kapiloff; George Schett; Ingo Thievessen; Felix B Engel
• Keywords: Microtubule organizing center

The switch from centrosomal microtubule-organizing centers (MTOCs) to non-centrosomal MTOCs during differentiation is poorly understood. Here, we identify AKAP6 as key component of the nuclear envelope MTOC. In rat cardiomyocytes, anchors proteins through its spectrin repeats, acting an adaptor between nesprin-1α and Pcnt or AKAP9. addition, AKAP9 form a protein platform tethering Golgi nucleus. Both exhibit MTOC activity utilizing either AKAP9, Pcnt-AKAP9, respectively. also required for formation in human osteoclasts. Moreover, ectopic expression epithelial cells sufficient recruit endogenous proteins. Finally, cardiomyocyte hypertrophy osteoclast bone resorption activity. Collectively, decipher at bi-layered structure generating two pools microtubules with organizer.