Stem cell transplantation rescued a primary open-angle glaucoma mouse model

• DOI: 10.7554/elife.63677
• Publication Date: 2021-01-28T13:23:38Z
• Authors: Siqi Xiong; Ajay Kumar; Shenghe Tian; Eman E Taher; Enzhi Yang; Paul R Kinchington; Xiaobo Xia; Yiqin Du
• Keywords: Myocilin

Glaucoma is a leading cause of irreversible blindness. In this study, we investigated if transplanted stem cells are able to rescue glaucoma mouse model with transgenic myocilin Y437H mutation and explored the possible mechanisms. Human trabecular meshwork (TMSCs) were intracamerally which reduced intraocular pressure, increased outflow facility, protected retinal ganglion preserved their function. TMSC transplantation also significantly TM cellularity, promoted secretion from into aqueous humor reduce endoplasmic reticulum stress, repaired tissue extracellular matrix modulation ultrastructural restoration. Co-culturing TMSCs mutant in vitro differentiating phagocytic functional cells. RNA sequencing revealed that had upregulated genes related regeneration neuroprotection. Our results uncovered therapeutic potential for curing elucidated mechanisms by achieve treatment effect.