Mechanically transduced immunosorbent assay to measure protein-protein interactions

• DOI: 10.7554/elife.67525
• Publication Date: 2021-09-28T10:00:16Z
• Authors: Christopher J Petell; Kathyrn Randene; Michael Pappas; Diego Sandoval; Brian D Strahl; Joseph S Harrison; Joshua P Steimel
• Keywords: Affinities; Binding affinities

Measuring protein-protein interaction (PPI) affinities is fundamental to biochemistry. Yet, conventional methods rely upon the law of mass action and cannot measure many PPIs due a scarcity reagents limitations in measurable affinity ranges. Here, we present novel technique that leverages concept friction produce mechanical signal correlates binding potential. The mechanically transduced immunosorbent (METRIS) assay utilizes rolling magnetic probes PPI affinities. METRIS measures translational displacement protein-coated particles on protein-functionalized substrate. scales with effective induced by PPI, thus producing when event occurs. uses as little 20 pmols wide range while exhibiting high resolution sensitivity. We use several were previously inaccessible using traditional methods, providing new insights into epigenetic recognition.