DNA-RNA hybrids at DSBs interfere with repair by homologous recombination

• DOI: 10.7554/elife.69881
• Publication Date: 2021-07-08T12:00:33Z
• Authors: Pedro Ortega; José Antonio Méri...; Ana G Rondón; Belén Gómez-González; Andrés Aguilera
• Keywords: Non-homologous end joining

DNA double-strand breaks (DSBs) are the most harmful lesions and their repair is crucial for cell viability genome integrity. The readout of DSB may depend on whether DSBs occur at transcribed versus non-transcribed regions. Some studies have postulated that DNA-RNA hybrids form to promote recombinational repair, but others challenged this notion. To directly assess formed or interfere with we used plasmid chromosomal-based systems analysis DSB-induced recombination in